Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak
نویسندگان
چکیده
منابع مشابه
A Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cyprinid Herpesvirus 2 in Gibel Carp (Carassius auratus gibelio)
A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay for Cyprinid herpesvirus 2 (CyHV-2) detection in gibel carp was developed. Following cloning and sequencing of the putative DNA helicase gene of CyHV-2 isolate from China, a set of four specific primers was designed based on the sequence. The MgCl2 concentration and the reaction temperature were optimized to 6 mM, 64°C, r...
متن کاملDevelopment of a multiplex PCR method for the simultaneous detection of four myxosporeans infecting gibel carp Carassius auratus gibelio.
Gibel carp Carassius auratus gibelio (Bloch), a commercially important freshwater-cultured fish in China, is threatened by myxosporeans, particularly Thelohanellus wuhanensis, Myxobolus honghuensis, M. wulii and M. turpisrotundus. Here, we developed a multiplex PCR assay for simultaneous detection of these 4 myxosporeans. The specific primers for each species were designed based on the 28S rDNA...
متن کاملMyxobolus oralis sp. n. (Myxosporea: Bivalvulida) infecting the palate in the mouth of gibel carp Carassius auratus gibelio (Cypriniformes: Cyprinidae).
During a survey on the myxosporean fauna of gibel carp Carassius auratus gibelio (Bloch) in China, a species of Myxobolus Bütschli, 1882 that did not conform to any known species was found. The species is characterised by the presence of round to ellipsoidal plasmodia of 2.6-4.0 mm in diameter in the palate of host. Mature spores are obovate in frontal view and lemon-shaped in lateral view, wit...
متن کاملDiversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...
متن کاملDiversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...
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ژورنال
عنوان ژورنال: MicrobiologyOpen
سال: 2019
ISSN: 2045-8827,2045-8827
DOI: 10.1002/mbo3.907